Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.

DNA probes that hybridize to a mycobacterial insertion sequence, IS900, present in multiple copies in the genome of Mycobacterium paratuberculosis were found to be highly specific for M. paratuberculosis. DNA sequences derived from IS900 were used to prepare DNA primers for detection and identificat...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Vary, P H, Andersen, P R, Green, E, Hermon-Taylor, J, McFadden, J J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267840/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2351737/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.5.933-937.1990
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