Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.

DNA probes that hybridize to a mycobacterial insertion sequence, IS900, present in multiple copies in the genome of Mycobacterium paratuberculosis were found to be highly specific for M. paratuberculosis. DNA sequences derived from IS900 were used to prepare DNA primers for detection and identificat...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Vary, P H, Andersen, P R, Green, E, Hermon-Taylor, J, McFadden, J J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267840/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2351737/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.5.933-937.1990
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