Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine.

An improved method of preparing bovine urine samples was developed for the rapid, specific, and sensitive detection of Leptospira interrogans serovar hardjo (subtype hardjobovis) DNA by the polymerase chain reaction (PCR). A total of 100 leptospire-free cows, 4 experimentally infected cows, and 2 ne...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Gerritsen, M J, Olyhoek, T, Smits, M A, Bokhout, B A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270437/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1757552/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.12.2805-2808.1991
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