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Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine.

An improved method of preparing bovine urine samples was developed for the rapid, specific, and sensitive detection of Leptospira interrogans serovar hardjo (subtype hardjobovis) DNA by the polymerase chain reaction (PCR). A total of 100 leptospire-free cows, 4 experimentally infected cows, and 2 ne...

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Dades bibliogràfiques
Publicat a:J Clin Microbiol
Autors principals: Gerritsen, M J, Olyhoek, T, Smits, M A, Bokhout, B A
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1991
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270437/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1757552/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.12.2805-2808.1991
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