Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine.

An improved method of preparing bovine urine samples was developed for the rapid, specific, and sensitive detection of Leptospira interrogans serovar hardjo (subtype hardjobovis) DNA by the polymerase chain reaction (PCR). A total of 100 leptospire-free cows, 4 experimentally infected cows, and 2 ne...

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Dettagli Bibliografici

Pubblicato in:	J Clin Microbiol
Autori principali:	Gerritsen, M J, Olyhoek, T, Smits, M A, Bokhout, B A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270437/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1757552/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.12.2805-2808.1991
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