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Directed enzyme evolution: climbing fitness peaks one amino acid at a time

Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse. Activities on new substrates can be obtained by improving variants with b...

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Bibliografiske detaljer
Main Authors: Tracewell, Cara A, Arnold, Frances H
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2703427/
https://ncbi.nlm.nih.gov/pubmed/19249235
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cbpa.2009.01.017
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