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Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes.
By using biotinylated enterotoxin DNA probes, a method to detect enterotoxigenic Escherichia coli by colony hybridization was developed. The treatment of colonies on nitrocellulose membrane filters with proteinase K and Triton X-100 was essential for obtaining the specific hybridization. A total of...
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| Publicat a: | J Clin Microbiol |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1987
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269376/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822764/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.25.10.1962-1965.1987 |
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