Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes.

By using biotinylated enterotoxin DNA probes, a method to detect enterotoxigenic Escherichia coli by colony hybridization was developed. The treatment of colonies on nitrocellulose membrane filters with proteinase K and Triton X-100 was essential for obtaining the specific hybridization. A total of...

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Dettagli Bibliografici

Pubblicato in:	J Clin Microbiol
Autori principali:	Kirii, Y, Danbara, H, Komase, K, Arita, H, Yoshikawa, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1987
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269376/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822764/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.25.10.1962-1965.1987
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