Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes.

By using biotinylated enterotoxin DNA probes, a method to detect enterotoxigenic Escherichia coli by colony hybridization was developed. The treatment of colonies on nitrocellulose membrane filters with proteinase K and Triton X-100 was essential for obtaining the specific hybridization. A total of...

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Bibliografski detalji
Glavni autori: Kirii, Y, Danbara, H, Komase, K, Arita, H, Yoshikawa, M
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC269376/
https://ncbi.nlm.nih.gov/pubmed/2822764
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