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Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes.

By using biotinylated enterotoxin DNA probes, a method to detect enterotoxigenic Escherichia coli by colony hybridization was developed. The treatment of colonies on nitrocellulose membrane filters with proteinase K and Triton X-100 was essential for obtaining the specific hybridization. A total of...

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Bibliografische gegevens
Gepubliceerd in:J Clin Microbiol
Hoofdauteurs: Kirii, Y, Danbara, H, Komase, K, Arita, H, Yoshikawa, M
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1987
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269376/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2822764/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.25.10.1962-1965.1987
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