Rapid Molecular Genotyping and Clonal Complex Assignment of Staphylococcus aureus Isolates by PCR Coupled to Electrospray Ionization-Mass Spectrometry

We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (M...

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Detalhes bibliográficos
Main Authors: Hall, Thomas A., Sampath, Rangarajan, Blyn, Lawrence B., Ranken, Raymond, Ivy, Cristina, Melton, Rachael, Matthews, Heather, White, Neill, Li, Feng, Harpin, Vanessa, Ecker, David J., McDougal, Linda K., Limbago, Brandi, Ross, Tracy, Wolk, Donna M., Wysocki, Vicki, Carroll, Karen C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Epidemiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2691079/
https://ncbi.nlm.nih.gov/pubmed/19297593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02175-08
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