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Rapid Molecular Genotyping and Clonal Complex Assignment of Staphylococcus aureus Isolates by PCR Coupled to Electrospray Ionization-Mass Spectrometry

We describe a high-throughput assay using PCR coupled to electrospray ionization-mass spectrometry (PCR/ESI-MS) to determine the genotypes of Staphylococcus aureus isolates. The primer sets used in the PCR/ESI-MS assay were designed to amplify the same genes analyzed in multilocus sequence typing (M...

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Autors principals: Hall, Thomas A., Sampath, Rangarajan, Blyn, Lawrence B., Ranken, Raymond, Ivy, Cristina, Melton, Rachael, Matthews, Heather, White, Neill, Li, Feng, Harpin, Vanessa, Ecker, David J., McDougal, Linda K., Limbago, Brandi, Ross, Tracy, Wolk, Donna M., Wysocki, Vicki, Carroll, Karen C.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2691079/
https://ncbi.nlm.nih.gov/pubmed/19297593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02175-08
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