Concurrent Serotyping and Genotyping of Pneumococci by Use of PCR and Electrospray Ionization Mass Spectrometry

A pneumococcal serotyping/genotyping system (PSGS) was developed based upon targeted PCR, followed by electrospray ionization mass spectrometry and amplicon base composition analysis. Eight multiplex PCRs, 32 targeting serotype-determining capsular biosynthetic loci, and 8 targeting multilocus seque...

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Detalhes bibliográficos
Main Authors: Massire, Christian, Gertz, Robert E., Svoboda, Pavel, Levert, Keith, Reed, Matthew S., Pohl, Jan, Kreft, Rachel, Li, Feng, White, Neill, Ranken, Ray, Blyn, Larry B., Ecker, David J., Sampath, Rangarajan, Beall, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3372165/
https://ncbi.nlm.nih.gov/pubmed/22442313
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.06735-11
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