Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization.
A novel chemical method was used to prepare biotin-labeled nucleic acids for nonisotopic hybridization. The method involves the transamination of unpaired cytosine residues in polynucleotides with sodium bisulfite and ethylenediamine. Primary amino groups on the cytosine derivatives are then reacted...
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| 發表在: | J Clin Microbiol |
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| Principais autores: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1986
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC268633/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2939103/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.23.2.311-317.1986 |
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