Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization.
A novel chemical method was used to prepare biotin-labeled nucleic acids for nonisotopic hybridization. The method involves the transamination of unpaired cytosine residues in polynucleotides with sodium bisulfite and ethylenediamine. Primary amino groups on the cytosine derivatives are then reacted...
Enregistré dans:
| Publié dans: | J Clin Microbiol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1986
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC268633/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2939103/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.23.2.311-317.1986 |
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