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Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid
This work presents an assay for total thiols and total disulfides in biological samples via HPLC quantification of 5-thio-2-nitrobenzoic acid (TNB) derived from the reaction of thiols with 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman’s reagent). This method also provides simultaneous quantifica...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2684446/ https://ncbi.nlm.nih.gov/pubmed/18926658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jpba.2008.08.033 |
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