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Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid

This work presents an assay for total thiols and total disulfides in biological samples via HPLC quantification of 5-thio-2-nitrobenzoic acid (TNB) derived from the reaction of thiols with 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman’s reagent). This method also provides simultaneous quantifica...

詳細記述

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書誌詳細
主要な著者: Chen, Wei, Zhao, Yong, Seefeldt, Teresa, Guan, Xiangming
フォーマット: Artigo
言語:Inglês
出版事項: 2008
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2684446/
https://ncbi.nlm.nih.gov/pubmed/18926658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jpba.2008.08.033
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