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Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid

This work presents an assay for total thiols and total disulfides in biological samples via HPLC quantification of 5-thio-2-nitrobenzoic acid (TNB) derived from the reaction of thiols with 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman’s reagent). This method also provides simultaneous quantifica...

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Bibliografiska uppgifter
Huvudupphovsmän: Chen, Wei, Zhao, Yong, Seefeldt, Teresa, Guan, Xiangming
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2008
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2684446/
https://ncbi.nlm.nih.gov/pubmed/18926658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jpba.2008.08.033
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