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A quantitative method for evaluating the stabilities of nucleic acids

We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of nucleic acid duplexes. We demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free energy differences bet...

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Bibliografiska uppgifter
Huvudupphovsmän: Gelfand, Craig A., Plum, G. Eric, Mielewczyk, Slawomir, Remeta, David P., Breslauer, Kenneth J.
Materialtyp: Artigo
Språk:Inglês
Publicerad: The National Academy of Sciences 1999
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC26844/
https://ncbi.nlm.nih.gov/pubmed/10339550
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