A quantitative method for evaluating the stabilities of nucleic acids

We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of nucleic acid duplexes. We demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free energy differences bet...

Full description

Saved in:
Bibliographic Details
Main Authors: Gelfand, Craig A., Plum, G. Eric, Mielewczyk, Slawomir, Remeta, David P., Breslauer, Kenneth J.
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences 1999
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC26844/
https://ncbi.nlm.nih.gov/pubmed/10339550
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items