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V-region mutation in vitro, in vivo, and in silico reveal the importance of the enzymatic properties of AID and the sequence environment

The somatic hypermutation of Ig variable regions requires the activity of activation-induced cytidine deaminase (AID) which has previously been shown to preferentially deaminate WRC (W = A/T, R = A/G) motif hot spots in in vivo and in vitro assays. We compared mutation profiles of in vitro assays fo...

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Detalhes bibliográficos
Main Authors: MacCarthy, Thomas, Kalis, Susan L., Roa, Sergio, Pham, Phuong, Goodman, Myron F., Scharff, Matthew D., Bergman, Aviv
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2682541/
https://ncbi.nlm.nih.gov/pubmed/19443686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0903803106
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