A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

BACKGROUND: Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. FINDINGS: We describe here protocols which adapt baculovirus generation into 96-well format. CONCLUSION: The established meth...

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Detalles Bibliográficos
Main Authors: Kärkkäinen, Hanna-Riikka, Lesch, Hanna P, Määttä, Antti I, Toivanen, Pyry I, Mähönen, Anssi J, Roschier, Miia M, Airenne, Kari J, Laitinen, Olli H, Ylä-Herttuala, Seppo
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2009
Assuntos:
Technical Note
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2680411/
https://ncbi.nlm.nih.gov/pubmed/19389242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-63
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