Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.
A method was developed for the purification of rotavirus RNA from fecal extracts in order to permit the sensitive identification of group A rotavirus in fecal specimens by the polymerase chain reaction. Sequential reactions with reverse transcriptase and Taq polymerase with directed primers from rot...
Uloženo v:
| Vydáno v: | J Clin Microbiol |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1990
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267924/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1696283/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.6.1300-1307.1990 |
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