Detection of group B and C rotaviruses by polymerase chain reaction.

We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and C rotaviruses and introduced a simple and convenient technique to purify viral RNA from stool specimens. Double-stranded RNA present in stool extracts was purified by adsorption to hydroxyapatite and was used as...

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Hauptverfasser: Gouvea, V, Allen, J R, Glass, R I, Fang, Z Y, Bremont, M, Cohen, J, McCrae, M A, Saif, L J, Sinarachatanant, P, Caul, E O
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC269811/
https://ncbi.nlm.nih.gov/pubmed/1645368
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