Detection of group B and C rotaviruses by polymerase chain reaction.
We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and C rotaviruses and introduced a simple and convenient technique to purify viral RNA from stool specimens. Double-stranded RNA present in stool extracts was purified by adsorption to hydroxyapatite and was used as...
Uloženo v:
| Vydáno v: | J Clin Microbiol |
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| Hlavní autoři: | , , , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1991
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269811/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1645368/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.3.519-523.1991 |
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