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Detection of group B and C rotaviruses by polymerase chain reaction.

We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and C rotaviruses and introduced a simple and convenient technique to purify viral RNA from stool specimens. Double-stranded RNA present in stool extracts was purified by adsorption to hydroxyapatite and was used as...

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Podrobná bibliografie
Vydáno v:J Clin Microbiol
Hlavní autoři: Gouvea, V, Allen, J R, Glass, R I, Fang, Z Y, Bremont, M, Cohen, J, McCrae, M A, Saif, L J, Sinarachatanant, P, Caul, E O
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269811/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1645368/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.3.519-523.1991
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