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Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.

A method was developed for the purification of rotavirus RNA from fecal extracts in order to permit the sensitive identification of group A rotavirus in fecal specimens by the polymerase chain reaction. Sequential reactions with reverse transcriptase and Taq polymerase with directed primers from rot...

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Dades bibliogràfiques
Publicat a:J Clin Microbiol
Autors principals: Wilde, J, Eiden, J, Yolken, R
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267924/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1696283/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.6.1300-1307.1990
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