載入...
Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the use of type IIs restriction enzymes and is performed by sim...
Na minha lista:
| Main Authors: | , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Public Library of Science
2009
|
| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2677662/ https://ncbi.nlm.nih.gov/pubmed/19436741 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0005553 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|