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Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the use of type IIs restriction enzymes and is performed by sim...

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Sonraí Bibleagrafaíochta
Main Authors: Engler, Carola, Gruetzner, Ramona, Kandzia, Romy, Marillonnet, Sylvestre
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2009
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2677662/
https://ncbi.nlm.nih.gov/pubmed/19436741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0005553
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