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A One Pot, One Step, Precision Cloning Method with High Throughput Capability
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed protein. We have devised a simple and rapid subclon...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Public Library of Science
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2574415/ https://ncbi.nlm.nih.gov/pubmed/18985154 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0003647 |
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