Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction.
We applied the polymerase chain reaction to detection of the pathogenic protozoan Toxoplasma gondii based on our identification of a 35-fold-repetitive gene (the B1 gene) as a target. Using this procedure, we were able to amplify and detect the DNA of a single organism directly from a crude cell lys...
Guardado en:
| Publicado en: | J Clin Microbiol |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1989
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267672/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2768467/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.8.1787-1792.1989 |
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