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Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction.

We applied the polymerase chain reaction to detection of the pathogenic protozoan Toxoplasma gondii based on our identification of a 35-fold-repetitive gene (the B1 gene) as a target. Using this procedure, we were able to amplify and detect the DNA of a single organism directly from a crude cell lys...

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Bibliografiske detaljer
Main Authors: Burg, J L, Grover, C M, Pouletty, P, Boothroyd, J C
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC267672/
https://ncbi.nlm.nih.gov/pubmed/2768467
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