Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction.

We applied the polymerase chain reaction to detection of the pathogenic protozoan Toxoplasma gondii based on our identification of a 35-fold-repetitive gene (the B1 gene) as a target. Using this procedure, we were able to amplify and detect the DNA of a single organism directly from a crude cell lys...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Burg, J L, Grover, C M, Pouletty, P, Boothroyd, J C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1989
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267672/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2768467/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.8.1787-1792.1989
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