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Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde
The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2672816/ https://ncbi.nlm.nih.gov/pubmed/19390151 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444909008324 |
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