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Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde

The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and...

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Detalhes bibliográficos
Main Authors: Vetting, Matthew W., Hegde, Subray S., Blanchard, John S.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2672816/
https://ncbi.nlm.nih.gov/pubmed/19390151
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444909008324
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