Loading...

Structure and function in rhodopsin: Kinetic studies of retinal binding to purified opsin mutants in defined phospholipid–detergent mixtures serve as probes of the retinal binding pocket

In the current standard procedure for preparation of mammalian rhodopsin mutants, transfected COS-1 cells expressing the mutant opsin genes are treated with 5 μM 11-cis-retinal before detergent solubilization for purification. We found that binding of 11-cis-retinal to opsin mutants with single amin...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Main Authors: Reeves, Philip J., Hwa, John, Khorana, H. Gobind
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC26713/
https://ncbi.nlm.nih.gov/pubmed/10051571
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!