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Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segment...

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Autors principals: Karch, H, Meyer, T
Format: Artigo
Idioma:Inglês
Publicat: 1989
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC267121/
https://ncbi.nlm.nih.gov/pubmed/2687320
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