Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segment...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Karch, H, Meyer, T
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1989
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267121/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2687320/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.12.2751-2757.1989
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