Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction.

In an effort to explore a sensitive taxon-specific detection system for bacteria, we sequenced the 16S rRNA from two strains of Rickettsia rickettsii, five other rickettsiae, and Coxiella burnetti to find a probe site unique to R. rickettsii. We then synthesized a 16-mer that hybridized only to the...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Wilson, K H, Blitchington, R, Shah, P, McDonald, G, Gilmore, R D, Mallavia, L P
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1989
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC267110/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2512323/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.27.12.2692-2696.1989
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