Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction.

In an effort to explore a sensitive taxon-specific detection system for bacteria, we sequenced the 16S rRNA from two strains of Rickettsia rickettsii, five other rickettsiae, and Coxiella burnetti to find a probe site unique to R. rickettsii. We then synthesized a 16-mer that hybridized only to the...

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Main Authors: Wilson, K H, Blitchington, R, Shah, P, McDonald, G, Gilmore, R D, Mallavia, L P
Format: Artigo
Jezik:Inglês
Izdano: 1989
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC267110/
https://ncbi.nlm.nih.gov/pubmed/2512323
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