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Direct Measurement of the Ionization State of an Essential Guanine in the Hairpin Ribozyme
Active site guanines are critical for self-cleavage reactions of several ribozymes, but their precise function(s) in catalysis are unclear. To learn whether protonated or deprotonated forms of guanine predominate in the active site, microscopic pK(a) values were determined for ionization of 8-azagua...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2670934/ https://ncbi.nlm.nih.gov/pubmed/19330013 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.156 |
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