Small-scale, semi-automated purification of eukaryotic proteins for structure determination

A simple approach that allows cost-effective automated purification of recombinant proteins in levels sufficient for functional characterization or structural studies is described. Studies with four human stem cell proteins, an engineered version of green fluorescent protein, and other proteins are...

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Detalhes bibliográficos
Main Authors: Frederick, Ronnie O., Bergeman, Lai, Blommel, Paul G., Bailey, Lucas J., McCoy, Jason G., Song, Jikui, Meske, Louise, Bingman, Craig A., Riters, Megan, Dillon, Nicholas A., Kunert, John, Yoon, Jung Whan, Lim, Ahyoung, Cassidy, Michael, Bunge, Jason, Aceti, David J., Primm, John G., Markley, John L., Phillips, George N., Fox, Brian G.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2668602/
https://ncbi.nlm.nih.gov/pubmed/17985212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-007-9032-5
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