Detection of bacteria by hybridization of rRNA with DNA-latex and immunodetection of hybrids.

A novel nucleic acid hybridization assay with a DNA probe immobilized on 1.25-micron-diameter latex particles was developed. Hybridization of the immobilized probe DNA with sample rRNA was complete in 10 to 15 min. Alkaline phosphatase-labeled anti-DNA-RNA was allowed to bind to the DNA-RNA hybrids...

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Udgivet i:J Clin Microbiol
Main Authors: Miller, C A, Patterson, W L, Johnson, P K, Swartzell, C T, Wogoman, F, Albarella, J P, Carrico, R J
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC266591/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2457597/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.26.7.1271-1276.1988
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