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Improvement of crystal quality by surface mutations of β-lactamase Toho-1

The β-lactamase Toho-1 exhibits a strong tendency to form merohedrally twinned crystals. Here, the crystal quality of Toho-1 was improved by using surface modification to remove a sulfate ion involved in crystal packing. The surface-modified Toho-1 variant (R274N/R276N) was crystallized under simila...

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Autori principali: Shimamura, Tatsuro, Nitanai, Yasushi, Uchiyama, Takuro, Matsuzawa, Hiroshi
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2664765/
https://ncbi.nlm.nih.gov/pubmed/19342785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109008240
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