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Neutron and X-ray Crystal Structures of a Perdeuterated Enzyme Inhibitor Complex Reveal the Catalytic Proton Network of the Toho-1 β-Lactamase for the Acylation Reaction

The mechanism by which class A β-lactamases hydrolyze β-lactam antibiotics has been the subject of intensive investigation using many different experimental techniques. Here, we report on the novel use of both neutron and high resolution x-ray diffraction to help elucidate the identity of the cataly...

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Autors principals: Tomanicek, Stephen J., Standaert, Robert F., Weiss, Kevin L., Ostermann, Andreas, Schrader, Tobias E., Ng, Joseph D., Coates, Leighton
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3576076/
https://ncbi.nlm.nih.gov/pubmed/23255594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.436238
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