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A Heavy-Atom Isotope Effect and Kinetic Investigation of the Hydrolysis of Semicarbazide by Urease from Jack Bean (Canavalia ensiformis)

A kinetic investigation of the hydrolysis of semicarbazide by urease gives a relatively flat log V/K vs. pH plot between pH 5 and 8. A log V(m) vs. pH plot shows a shift of the optimum V(m) toward lower pH when compared to urea. These results are explained in terms of the binding of the outer-N of t...

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Detalhes bibliográficos
Main Authors: Marlier, John F., Fogle, Emily J., Cleland, W.W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663901/
https://ncbi.nlm.nih.gov/pubmed/18817416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi801338c
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