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Purification and Properties of Urease Derived from Hydrated Seeds of Jack Bean, Canavalia ensiformis (L) DC.
Urease from jack bean meal and hydrated seeds has been obtained in 25 to 33% yield with specific activity in the range of 1000 to 1070 units/mg protein. A purification of 100 to 130-fold was achieved from meal and fully soaked seeds. Use of β-mercaptoethanol and EDTA was found essential to obtain th...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1966
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1086385/ https://ncbi.nlm.nih.gov/pubmed/16656289 |
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