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Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature

BACKGROUNDS: Whole genome amplification (WGA) is a practical solution to eliminate molecular analysis limitations associated with genomic DNA (gDNA) quantity. Different methods have been developed to amplify the whole genome, including primer extension preamplification (PEP), degenerate oligonucleot...

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Detalhes bibliográficos
Main Authors: Alsmadi, Osama, Alkayal, Fadi, Monies, Dorota, Meyer, Brian F
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2663774/
https://ncbi.nlm.nih.gov/pubmed/19309528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-48
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