Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR

BACKGROUND: Phi29 polymerase based amplification methods provides amplified DNA with minimal changes in sequence and relative abundance for many biomedical applications. RNA virus detection using microarrays, however, can present a challenge because phi29 DNA polymerase cannot amplify RNA nor small...

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Detalhes bibliográficos
Main Authors: Berthet, Nicolas, Reinhardt, Anita K, Leclercq, India, van Ooyen, Sven, Batéjat, Christophe, Dickinson, Philip, Stamboliyska, Rayna, Old, Iain G, Kong, Katherine A, Dacheux, Laurent, Bourhy, Hervé, Kennedy, Giulia C, Korfhage, Christian, Cole, Stewart T, Manuguerra, Jean-Claude
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2008
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2535778/
https://ncbi.nlm.nih.gov/pubmed/18771595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-9-77
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