Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species.

Sequence data on Helicobacter pylori 16S rRNA were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (PCR) for detection of H. pylori. H. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. Reverse transcription of rRNA was followed...

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Bibliographic Details
Main Authors: Engstrand, L, Nguyen, A M, Graham, D Y, el-Zaatari, F A
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC265495/
https://ncbi.nlm.nih.gov/pubmed/1383268
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