Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species.

Sequence data on Helicobacter pylori 16S rRNA were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (PCR) for detection of H. pylori. H. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. Reverse transcription of rRNA was followed...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Engstrand, L, Nguyen, A M, Graham, D Y, el-Zaatari, F A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265495/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1383268/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.9.2295-2301.1992
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados