Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species.

Sequence data on Helicobacter pylori 16S rRNA were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (PCR) for detection of H. pylori. H. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. Reverse transcription of rRNA was followed...

Description complète

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Engstrand, L, Nguyen, A M, Graham, D Y, el-Zaatari, F A
Format: Artigo
Langue:Inglês
Publié: 1992
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC265495/
https://ncbi.nlm.nih.gov/pubmed/1383268
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires