Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species.

Sequence data on Helicobacter pylori 16S rRNA were used to select two 22-base oligonucleotide primers for use in a polymerase chain reaction (PCR) for detection of H. pylori. H. pylori cells were treated with lysis buffer, boiled, and chloroform extracted. Reverse transcription of rRNA was followed...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Main Authors: Engstrand, L, Nguyen, A M, Graham, D Y, el-Zaatari, F A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265495/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1383268/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.9.2295-2301.1992
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