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Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction.

Several simplified methods for treating mycobacteria to release DNA for amplification by the polymerase chain reaction (PCR) were investigated. The most effective of the methods was sonication. Samples were placed in screw-capped microcentrifuge tubes that were then placed in a plastic rack. The rac...

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Bibliografski detalji
Izdano u:J Clin Microbiol
Glavni autori: Buck, G E, O'Hara, L C, Summersgill, J T
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 1992
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC265279/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1583142/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.5.1331-1334.1992
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